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OBJECTIVE:To study chemical compounds of Monoraphidium dybowskii,and to investigate the in vitro antibacte-rial and antioxidant activities of isolated compounds. METHODS:The ethanol extract of M. dybowskii were extracted with aether petrolei,ethyl acetate and n-butyl alcohol. The ethyl acetate extract was separated from M. dybowskii and chemical components were analyzed by sillica gel column chromatogram,HPLC and GC-MS. Their structures were identified according to physicochemi-cal properties and NMR. MIC of 4 isolated compounds to Pseudomonas aeruginosa,Candida albicans,Bacillus subtilis and Esche-richia coli were determined by resazurin disc test. Free radical scavenging rate(concluated by IC50)and reducing capacity were mea-sured by 1,1-diphenyl-2-picryl-diazanyl (DPPH) and ferric reducing antioxidant power (FRAP) assay. RESULTS:Compounds 1-6 were obtained from E4 and E5 segments of ethyl acetate extract of M. dybowskii,and their structures were identified as stigmas-terol,diisonoyladipate,indole-3-carboxylic acid,(+)-epiloliolide,(-)-loliolide,5-hydroxy-3,4-dimethy-5-pentyl-2(5H)-furanone. MIC of compounds 3-6 were 10-500 μg/mL,and IC50 ranged 22.02-71.01 μg/mL;FRAP ranged (62.04 ± 5.36)-(281.22 ± 8.3) μmol/L. CONCLUSIONS:M. dybowskii contains multiple lipid and alkanoic acid,and possesses certain in vitro antibacterial and antioxidant activities.
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Objective To study the protective earth requirement for touched metal parts of medical electrical equipment to improve the manufacturer's understanding on national standards.Methods The isolation methods for the touched metal parts in national standards were studied,and the principle of protective earth combined with insulation separating was analyzed.The characteristics of the second isolation method was discussed,and the respiratory system structure and electroshock protection of the anaesthetic machine were taken as examples.Results The touched metal parts with no measures for grounding,double isolation or reinforced insulation was determined by structure detection to meet the requirements of national standards in case basic insulation ceased to be effective and the parts were uncharged.Conclusion The necessities to girt the touched metal parts with measures for grounding,double isolation or reinforced insulation should depend on the understanding on national standards and electrical construction of the equipment under test by structure detection.
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Abstract BACKGROUND: Many cytokines can be detected in saliva and serum, and have more clinical significance in the diagnosis, prognosis and treatment of oral mucosa disease. OBJECTIVE: To compare the interferon-γ and interleukin-10 levels in serum and saliva of patients with different types of oral lichen planus and to explore the feasibility of saliva samples as a substitute of blood samples to study the interferon-γ and interleukin-10 levels in serum and saliva. METHODS:Totaly 45 patients with oral lichen planus admitted at the Department of Periodontology, the Stomatological Hospital of Jiamusi University from January to July 2014 were enroled, including 15 cases of erosion type (erosion group), 15 cases of congestive erythema (congestive erythema group) and 15 cases of reticulate type (reticulate group). Another 15 healthy controls admitted for physical examination at the Department of Physical Examination, the Stomatological Hospital of Jiamusi University were enroled as controls. ELISA method was used to detect the interferon-γ and interleukin-10 levels in serum and saliva in the four groups. RESULTS AND CONCLUSION: Compared with the control group, the interferon-γ levels in serum and saliva were lower in the other three groups (P < 0.01), while there were significant differences in the interferon-γ level among the patients with different types of oral lichen planus (P < 0.01). The interleukin-10 levels in serum and saliva were significantly higher in the erosion group and congestive erythema group than those in the control group (P < 0.01 orP < 0.05) and reticulate groupP < 0.01 orP < 0.05). Experimental findings suggest that the levels of interferon-γ and interleukin-10 in serum and saliva are highly correlated in patients with different types of oral lichen planus, and saliva samples can be instead of blood samples to detect the levels of interferon-γ and interleukin-10 in patients with oral lichen planus.
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BACKGROUND:The micro-arc oxidation technology can enhance the corrosion resistance of magnesium and its aloys to improve the biological properties of the surface. OBJECTIVE: In order to regulate the biological activity of medical pure magnesium, to modify the micro-arc oxidation coating by adding nano-SiO2 and nano-TiO2 into the plating solution, and to study the biological coating effects on osteoblast proliferation and differentiation. METHODS: Round magnesium sheets were divided into three groups, respectively treated with silicate electrolyte containing 7.5 g/L nano-SiO2 (nano-SiO2 group), 4.8 g/L nano-TiO2 (nano-TiO2 group), and nothing (control group). Passage 3 osteoblasts were seeded onto the specimen surface in the three groups to observe the early morphology, proliferation and alkaline phosphatase activity of osteoblasts. RESULTS AND CONCLUSION:Osteoblasts grew wel in the nano-SiO2 group and nano-TiO2 group, presenting with clear outline, and the cels were long spindle- and polygon-shaped. But in the control group, the cels grew poor in the specimen surface. Cel counting kit-8 test showed that the absorbance values and alkaline phosphatase activities were increased with time in the three groups. Compared with the control group, the proliferative activity was higher in the nano-SiO2 group and nano-TiO2 group at days 1, 3, 5 after seeding, while the activity of alkaline phosphatase was also higher in the nano-SiO2 group and nano-TiO2 group at days 3, 5 after seeding. These findings indicate the micro-arc oxidation coatings containing nano-SiO2and nano-TiO2 can promote osteoblast proliferation and osteogenetic activity, which have good biocompatibility.
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BACKGROUND:Various studies confirm that smoking can contribute to the resorption of periodontal alveolar bone. OBJECTIVE:To observe the effect of smoking in alveolar bone resorption of periodontitis rat models. METHODS:A total of 24 rats were randomly divided into three groups. Normal group:rats were normal y fed without any other pre-treatment;Control group:experimental periodontitis model was established using wire ligation method in rats;Experimental group:rat models were given passive smoking during experimental modeling period. Al rats were sacrificed after 8 weeks of modeling, periodontal tissue were removed. Hematoxylin-eosin staining was used for examining pathological changes in periodontal tissue, and immunohistochemical analysis was done for observing receptor activator of nuclear factor-κB ligand and osteoprotegrin expression. RESULTS AND CONCLUSION:After 8 weeks of modeling, expression of receptor activator of nuclear factor-κB ligand factor was significantly higher in alveolar bone of rats from experimental group in comparison to control group (P<0.05), whereas expression of osteoprotegerin in alveolar bone was significantly greater in rats from control group when compared to experimental group (P<0.05). This finding suggests that smoking can increase the expression of receptor activator of nuclear factor-κB ligand protein and reduce osteoprotegrin expression in periodontal rats, thus increasing the resorption of periodontal alveolar bone.
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BACKGROUND:In recent years, many drugs emerge to control tooth movement, and scholars in China begin to investigate Chinese herbs with moderate nature and smal adverse reaction. OBJECTIVE:To observe the relapse after orthodontic tooth movement, osteoprotegerin and osteoclast differentiation factor expression in periodontal tissue after rats were treated with local tanshinone type IIA at different doses. METHODS:A total of 48 male Wistar rats were randomly divided into four groups:control, low dose (tanshinone type IIA 0.36 mg/d), medium dose (tanshinone type IIA 0.72 mg/d), and high dose (tanshinone type IIA 1.44 mg/d) groups. Taking anterior teeth as the anchorage, the maxil ary first molar of rats was tracted to mesial movement. In experimental groups, gingival mucosa of the first molar was local injected with tanshinone type IIA 1 day before the force device was removed, while control group was injected with physiological saline, once a day, for 4 weeks. Immediately, 1 week, and 4 weeks after the force device was removed, the distance between the maxil ary first molar and second molar was measured and body mass was weighted. The animals were kil ed after 4 weeks, osteoprotegerin and osteoclast differentiation factor expression in maxil ary first molar and periodontal tissue were determined using immunohistochemical staining. RESULTS AND CONCLUSION:There was no obvious change in the body weight of rats in each group (P>0.05). In low, medium and high dose groups, recurrent distance of the teeth was shorter than that in control group (P smal er the degree of recurrence was. Osteoprotegerin expression in the periodontal tissue was significantly higher in the experimental groups than the control group (P<0.05), while osteoclast differentiation factor expression was significantly lower than the control group (P<0.05). The ratio of osteoprotegerin/osteoclast differentiation factor in the periodontal tissue was greater than 1 in both control group and experimental groups, and reached the peak in the high dose group. Local delivery of tanshinone type IIA has no impact on body weight of normal rats, and can effectively control the recurrence rate after orthodontic tooth movement. Within a certain range, high dose achieves the most obvious effect. Regulating osteoclast through adjusting the ratio of osteoprotegerin/osteoclast differentiation factor could be the molecular mechanism of tanshinone type IIA accelerating the periodontal tissue rebuilding.
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10.3969/j.issn.2095-4344.2013.24.013
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Objective To compare the three-dimensional eephalometric and three-dimensional CT measurements of adult zygomatic complex among a Sanjiang population.Methods 120 female natives of Sanjiang region from Chinese Han between 19 to 23 years of age (mean 21.4 years),were randomly selected from students of Jiamusi University.Three-dimensional cephalometric and CT measurements of the face and skull were taken,with reference to zygomatic size measurement by Prof.Qi Zuo-liang,for the comparative study, which included the upper,mid and lower facial width followed by the length,width and angle of malar process,and width of the facial bone.Statistical analysis was done with the obtained measurements.Results Three-dimensional CT analysis showed facial width ratio of 0.83 and 0.79 and skeletal facial profile width ratio of 0.81 and 0.77,respectively,when compared with three-dimensional cephalometric analysis that showed facial width ratio of 0.84 and 0.77.Both values showed no statistical significance (P>0.05).Conclusion Three-dimensional CT measurement as the same to three-dimensional cephalometric can be used in the diagnosis of prominent malar complex.
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Objective To study the effects of alternating magnetic field with different intensities on the proliferation of human squa-mous tongue cancer cells in vitro. Methods Viable cells with the OD value cell and flow cytometry were revealed through MTT assay to evaluate the proliferation and apeptosis and cell cycle respectively after the cells were exposed to electromagnetic fields of different intensity (5mT,8mT,11mT) once per day lasting 1 hour for 3 days. The sham -exposure controls were correspondingly established. Results We compared the electromagnetic field groups with the normal groups by MTT assay after 24,48,72 hours. By analyzing the data in SPSS sta-tistical software , we found that the OD value of electromagnetic field groups was significantly less than that of the control groups (P <0.01) . The rates of apoptosis cells by flow cytometry revealed that EMF groups had no change as compared with control groups. But the cell cycle displayed significant chang at 0.5rot. Conclusion The cells displayed significant changes with obvious Tca8113 cell prolifera-tion inhibition and hold - up cell cycle after being exposed to alternating magnetic field of different intensity. But human squamous tongue cancer ceils could not be induced to apeptosis.
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Objective In this research,the relationship between blood estrogen level and salivary glands of female rat was studied.Methods Thirty-two female Wister rats were randomly divided into four groups:control group,sham operation group,ovariectomized and injected estradiol benzoate group and ovariectomized group.Menopausal rat model was established by ovariectomy.By testing E2,histological examination and examing Cu,Zn-SOD expression through immunochemical staining,we studied the effects of estrogen decreasing on female rat salivary.Results Compared with control group,sham operation group,ovariectomized and injected estradiol benzoate group,a significant decrease in scrum estrogen level was observed in ovariectomized group(P